High-performance Liquid Chromatographic Method for the Quantification of Gallic Acid in Simhanada Guggulu


Shilpa Jain1, Neha Jain2*, Mohan Lal Kori2, Abhishek Kumar Jain3

1Sagar Institute of Pharmaceutical sciences, Sagar, 470002, M. P., India,

2Vedica College of B. Pharmacy, R.K.D.F. University, Bhopal, 462037, M.P., India

3Sagar Institute of Research Technology & Science – Pharmacy, Bhopal, 462037, M.P., India


Marker compounds quantification with new analytical tools and methods is necessary for establishing the authenticity and usage of Ayurvedic or herbal formulations. Simhanada guggulu or guggul is one of the supportive Ayurvedic medicines for treatment of rheumatoid arthritis and used in various disorders in Ayurveda such as limping, anemia, gout, disease of skin, cough, abdominal lump, pain digestive impairment. Simhanada guggulu is an Ayurvedic herbal formulation made by some selected herbs. The rejuvenating and tonic properties of ‘Simhanada guggulu’ are considered majorly due to their antioxidant principles, which in turn is due to the presence of phenolic compounds. A high-performance liquid chromatography (HPLC) method has been developed for quantitative determination of the gallic acid in ‘Simhanada guggulu’. The acidic mobile phase used in RP18 column which enabled efficient separation of gallic acid. A binary gradient with mobile phase containing solvent A (Acetonitrile) and solvent B (water: 0.3% O-Phosphoric Acid) was used for analysis. Elution was carried out at flow rate of 0.8 mL/min. Pure gallic acid Rt was found to be 5.29 min and peak with same Rt was also observed in prepared formulation. Gallic acid content of prepared formulation was found to be 2.28 %. The developed HPLC-UV method is simple, rapid and help as tool for the standardization of Simhanda guggulu.


Meta-analysis to Assess Role of Systemic Antibiotics in Root Canal Treatment


Nouman Noor, Sadaf Humayoun, Humaira Zafar*, Noor Khan Lakhnana, Kiran Tauseef Bukhari

Al Nafees Med College, Isra University, Islamabad Campus, Pakistan


The role of systemic antibiotics in root canal treatment (RCT) always remained controversial. To exactly identify the state of affairs, regarding whether or not the usage of systemic antibiotics in RCT, the current meta analysis was carried out. To identify the frequency of discouraging or preferring the use of systemic antibiotics in RCT. Secondly to identify the efficacy of various antibiotics in post RCT infections. Department of Operative Dentistry, Rawal Dental College Islamabad and Pathology Department of Al Nafees Medical College & Hospital, Isra University Islamabad Campus, Pakistan. Total 41 published studies in 03 and a half decades were included in the study i.e 1981 – 2017. Various authentic electronic sources were used to gather adequate and authentic data by simple random sampling technique. 66.6%(n=08) studies from 1987 till 2011, were not in favour of using systemic antibiotics as a part of RCT. While 33.3% (n=04) preferred using systemic antibiotics for RCT. Regarding the local antibiotic preference, 33.3% (n=04) studies were in favour of using a combination of triple antibiotic paste comprising of metronidazole, minocycline and ciprofloxacin. There is no role of systemic/local antibiotics in endodontic management. However, the use of antibiotics is only recommended if deemed necessary by viewing the premorbid of patient.


Therapeutic Properties and Nutritive Values of Some Fruit Bearing Medicinal Plants of Rajasthan State in India


Gupta GK1, Tejas Joshi1, Keshu Madhudiya1, Arijit Chaudhuri2* 

Department of Agriculture, Madhav University, Sirohi-307 026, Rajasthan, India

Department of Pharmacy, Manav Bharti University, Solan-173229, Himachal Pradesh, India


The medicinal plants imparts chief role in protecting our health from various disease. It is nature’s gift to human being to live healthy life. Medicinal plants are believed to be much safer and proved as elixir in the treatment of various ailments. Medicinal plants used in Indian system of medicine from Rajasthan state have been surveyed and categorized systematically. The manuscript incorporated the therapeutic properties and nutritive values of medicinal plants of Rajasthan. The paper deals with 11 medicinal plants, thoroughly indexed along with their important traditional application for the cure of various ailments. This study also incorporates the ethno-botany and biological activities of these important plants.


Catharanthus roseus Combination Therapy with Orthodox Oral Hypoglycemic Drugs: A Novel Approach to Diabetes Mellitus Treatment


Ohadoma, SC1*, Michael, HU2

1Department of Pharmacology, and Therapeutics, College of Medicine, Imo State University, PMB 2000, Owerri, Nigeria

2Department of Pharmacology and Toxicology, Madonna University, PMB 05, Elele, Rivers State, Nigeria


Most of the chronic diseases including diabetes mellitus are difficult to treat successfully with orthodox drugs. Investigation in complementary and alternative medicines is now being intensified to proffer lasting solution. Natural products and their derivatives may be considered as a potential source of novel compounds which can combine with orthodox drugs to enhance their hypoglycemic effect. To investigate the complementary and synergistic effect of Catharanthus roseus (C. roseus) leaves extract with oral hypoglycemic synthetic drugs sulphonylurea (glibenclamide) and biguanide (metformin). Experimental induction of diabetes in rats using alloxan model was employed. Five rats each of six groups were used. Group I received distilled water only. Groups II, III and IV received 250 mg/kg of methanol extract, 100 mg/kg of biguanide (metformin) and 1 mg/kg of sulphonylurea (glibenclamide), respectively. Groups V and VI received extract-metformin and extract-glibenclamide combination at doses as above, respectively. Administration of the six groups was carried out once daily for seven days. At 2, 12, 24, 72 and 168 h, fasting blood glucose was determined using a glucometer. When compared with control alone, all medicaments significantly (p<0.05) lowered blood glucose levels. The highest percentage reduction in blood glucose (64.9%) occurred with extract-biguanide (metformin) combination. The leaves extract of C. roseus – biguanide (metformin) combination offered a promising novel approach to diabetes mellitus treatment.


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