High-performance Liquid Chromatographic Method for the Quantification of Gallic Acid in Simhanada Guggulu

 

Shilpa Jain1, Neha Jain2*, Mohan Lal Kori2, Abhishek Kumar Jain3

1Sagar Institute of Pharmaceutical sciences, Sagar, 470002, M. P., India,

2Vedica College of B. Pharmacy, R.K.D.F. University, Bhopal, 462037, M.P., India

3Sagar Institute of Research Technology & Science – Pharmacy, Bhopal, 462037, M.P., India

Abstract

Marker compounds quantification with new analytical tools and methods is necessary for establishing the authenticity and usage of Ayurvedic or herbal formulations. Simhanada guggulu or guggul is one of the supportive Ayurvedic medicines for treatment of rheumatoid arthritis and used in various disorders in Ayurveda such as limping, anemia, gout, disease of skin, cough, abdominal lump, pain digestive impairment. Simhanada guggulu is an Ayurvedic herbal formulation made by some selected herbs. The rejuvenating and tonic properties of ‘Simhanada guggulu’ are considered majorly due to their antioxidant principles, which in turn is due to the presence of phenolic compounds. A high-performance liquid chromatography (HPLC) method has been developed for quantitative determination of the gallic acid in ‘Simhanada guggulu’. The acidic mobile phase used in RP18 column which enabled efficient separation of gallic acid. A binary gradient with mobile phase containing solvent A (Acetonitrile) and solvent B (water: 0.3% O-Phosphoric Acid) was used for analysis. Elution was carried out at flow rate of 0.8 mL/min. Pure gallic acid Rt was found to be 5.29 min and peak with same Rt was also observed in prepared formulation. Gallic acid content of prepared formulation was found to be 2.28 %. The developed HPLC-UV method is simple, rapid and help as tool for the standardization of Simhanda guggulu.

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Meta-analysis to Assess Role of Systemic Antibiotics in Root Canal Treatment

 

Nouman Noor, Sadaf Humayoun, Humaira Zafar*, Noor Khan Lakhnana, Kiran Tauseef Bukhari

Al Nafees Med College, Isra University, Islamabad Campus, Pakistan

Abstract

The role of systemic antibiotics in root canal treatment (RCT) always remained controversial. To exactly identify the state of affairs, regarding whether or not the usage of systemic antibiotics in RCT, the current meta analysis was carried out. To identify the frequency of discouraging or preferring the use of systemic antibiotics in RCT. Secondly to identify the efficacy of various antibiotics in post RCT infections. Department of Operative Dentistry, Rawal Dental College Islamabad and Pathology Department of Al Nafees Medical College & Hospital, Isra University Islamabad Campus, Pakistan. Total 41 published studies in 03 and a half decades were included in the study i.e 1981 – 2017. Various authentic electronic sources were used to gather adequate and authentic data by simple random sampling technique. 66.6%(n=08) studies from 1987 till 2011, were not in favour of using systemic antibiotics as a part of RCT. While 33.3% (n=04) preferred using systemic antibiotics for RCT. Regarding the local antibiotic preference, 33.3% (n=04) studies were in favour of using a combination of triple antibiotic paste comprising of metronidazole, minocycline and ciprofloxacin. There is no role of systemic/local antibiotics in endodontic management. However, the use of antibiotics is only recommended if deemed necessary by viewing the premorbid of patient.

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Preliminary Phyochemical Analysis and Characterization of Flavonoid Moiety from Vitex negundo Leaves Origin in Madhya Pradesh State by HPLC Study 

 

Firdous Ahmad Dar1*, Kirti Jain2, Bharti Jain3,  Madhuri Modak1

1Government Motilal Vigyan Mahavidyalaya,  Jahangirabad, Bhopal-462004, Madhya Pradesh, India

2Govt. Science and Commerce College Benazir, Jahangirabad, Bhopal-462008, Madhya Pradesh, India

3Sarojini Naidu Govt Girls PG Autonomous College, Bhopal (M.P.)-462016, India

Abstract

In the present study leaves of an important medicinal plant Vitex negundo was subjected to a phytochemical screening, flavonoid content and characterization of flavonoids moiety of Vitex negundo leaves extracts. The petroleum ether, chloroform, ethanol, methanol and aqueous extracts were prepared, and further screened for preliminary phytochemical test. The quantification of quercetin was investigated in ethanol extract by HPLC study. Preliminary phytochemical revealed the presence of flavonoids and phenolic compounds in ethanol, methanol and aqueous extracts of leaves of Vitex negundo. The ethanol, methanol and aqueous extracts investigated for antioxidant activity, and ethanol extract indicates the presence of highest quantity of flavonoids and polyphenol compared to other extracts. The HPLC chromatogram confirmed the flavonoids moiety was quercetin in ethanol extract, and the yield of quercetin was 42.18 ng/ml

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Therapeutic Properties and Nutritive Values of Some Fruit Bearing Medicinal Plants of Rajasthan State in India

 

Gupta GK1, Tejas Joshi1, Keshu Madhudiya1, Arijit Chaudhuri2* 

Department of Agriculture, Madhav University, Sirohi-307 026, Rajasthan, India

Department of Pharmacy, Manav Bharti University, Solan-173229, Himachal Pradesh, India

Abstract

The medicinal plants imparts chief role in protecting our health from various disease. It is nature’s gift to human being to live healthy life. Medicinal plants are believed to be much safer and proved as elixir in the treatment of various ailments. Medicinal plants used in Indian system of medicine from Rajasthan state have been surveyed and categorized systematically. The manuscript incorporated the therapeutic properties and nutritive values of medicinal plants of Rajasthan. The paper deals with 11 medicinal plants, thoroughly indexed along with their important traditional application for the cure of various ailments. This study also incorporates the ethno-botany and biological activities of these important plants.

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