Development and Validation of a Dissolution Method for Desloratadine Coated Tablets

 

Brunna Ricci Falcão, Letícia de Melo Teixeira, Francine Zachow Philippsen, Tiago Rafael Sausen*

1Curso de Farmácia, Pontifícia Universidade Católica do Paraná, campus Toledo, 85902–532, Brazil

Abstract

The aim of this work was the development and validation of a dissolution methodology for desloratadine-coated tablets by spectrophotometry on ultraviolet (UV). 0.1 M hydrochloric acid (HCl), pH 4.5-citrate buffer and pH 6.8 phosphate buffer were tested as dissolution medium. In addition, influences of apparatus, and rotation speed were evaluated. After an UV scan spectrum from 500 to 200 nm, to determine the maximum wavelength absorbance, samples were analyzed by UV visible spectrophotometric method. The parameters selected were 0.1 M HCl as dissolution medium, using paddles as apparatus at 50 rpm, with analysis at wavelength of 280 nm. The method was validated as per ICH guidelines, and the results showed that the dissolution methodology for desloratadine-coated tablets with 0.1 M HCl as dissolution medium, using paddles as apparatus at 50 rpm, with analysis at wavelength of 280 nm, with sampling points at 5, 10, 15 and 30 minutes is specific, linear, precise and accurate and could be applied for quality control of desloratadine tablets, since there is no official monograph.

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Protective Effect of Leaves of Ficus carica Against Carbon Tetrachloride-Induced Hepatic Damage in Rats 

 

Manik Sharma1, Abid R1*, Younus Ahmad2, Nelofar Ghulam Nabi3

1Department of Zoology, Bhoj Mahavidyalaya, Bhopal (M.P.)-462016, India

2S.S.L. Jain College, Vidisha, (M.P.)-464001, India

3Govt. Maharani Laxmi Bai Girls` P.G. (Autonomous) College, Bhopal (M.P.)-462002, India

Abstract

Fig leaves (Ficus carica Linn.) belonged to the family Moraceae and used as a source of medicines for the ailment of diseases. The present study was conducted to evaluate the hepatoprotective activity against the carbon tetrachloride induced toxic chemical in rats. The petroleum ether, ethyl acetate and methanol extract of Ficus carica leaves was prepared, and evaluated for phytochemical screening. The serum level of glutamic oxaloacetate transaminase (SGOT), glutamic pyruvic transaminase (SGPT) and bilirubin were investigated for the assessment of hepatoprotective activity of ethyl acetate extract. Additionally, the histological changes in liver were observed. Preliminary phytochemical investigations of the extracts of leaves of Ficus carica revealed the presence of flavonoids, tannins, phenolic compounds, alkaloids, glycosides, fats and carbohydrates. Pre-treatment with ethyl acetate extract of Ficus carica led to significant (p<0.05) decrease in serum SGOT, SGPT and bilirubin when compared to control group rats treated CCl4 in dose-dependent manner. The outcomes of histological study revealed that there was significant reversal of histological functional of liver. In conclusion, the findings of this study validated that the Ficus carica can improve CCl4-induced hepatotoxicity.  

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Comparative Effects of Quail Eggs, Quail Eggs/Vit C and Glibenclamide on Streptozocin-induced Diabetes mellitus 

 

Adebayo-Gege GI1, Seriki AS1*, Oguche BE1, Ojo RJ2 

1Department of Physiology, Faculty of Basic Medical Sciences, Bingham University, Karu-900110, Nigeria

2Department of Biochemistry, Faculty of Sciences, Bingham University, Karu-900110, Nigeria

Abstract

In recent years, quail eggs have become a subject of interest because of its properties and medical benefits. In this study, the separate and combined effects of quail eggs and vitamin C on the blood glucose level and body weight of streptozotocin-induced diabetic rats were investigated. The 30 wistar rats were randomly assigned into five groups; Group I (control), Group II (diabetic rats without treatment), Group III (diabetic rats treated with raw quail eggs only), Group IV (diabetic rats treated with raw quail eggs and vitamin C) and Group V (diabetic rats treated with Glibenclamide 600 µg/kg body weight). Diabetes mellitus was induced in fasted rats with a single intraperitoneal injection of streptozotocin (50 mg/kg body weight). The induced rats were then treated with the Quail eggs, Quail eggs/vit C, and Glibenclamide. At the end of the 10 days of treatment, the group treated with quail eggs and vitamin C at 0.15 ml/kg and 200 mg/kg body weight per day, respectively showed a significant (p< 0.05) hypoglycaemic effect, as the mean blood glucose level in the group decreased with an increase in the mean body weight. The group treated with Glibenclamide showed similar and stronger effect of hypoglycaemia. The group treated with quail eggs alone did not show any significant hypoglycaemic effect. Consequently, the groups treated quail eggs/vit C and Glibenclamide lost significant body weight. Quail eggs alone, quail eggs combined with vit C and Glibenclamide have hypoglycaemic effect after 10 days of administration, and could therefore be used to manage diabetes mellitus. Glibenclamide is however more potent for this purpose than quail egg/vit C, which is also more potent than quail egg alone. 

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