Conference Proceeding of National Seminar on Current Status and Future Scope for Nanomaterials and Nanotechnology in Drug Discovery and Development

 

Organized by Himachal Institute of Pharmaceutical Education and Research (HIPER), Nadaun Himachal Pradesh, India from 23-24 February 2018

Abstract

IInd National Seminaron “Current Status and Future Scope for Nanomaterials and Nanotechnology in Drug Discovery and Development” was organized by Himachal Institute of Pharmaceutical Education and Research (HIPER), Nadaun Himachal Pradesh, India from23-24 February 2018. The seminar was sponsored by Indian Council of Medical Research, New Delhi and Himachal Pradesh Technical University, Hamirpur. The conference invites all the participants across the various states of India to attend and share their insights and convey recent developments in the field of Pharmaceutical Nanotechnology. Nanotechnology seems to have gained a widespread interest in the recent years. This conference had a variety of keynote lectures, Oral Presentation, Poster Presentations, Scientific Quiz Competition and discussions with renowned speakers, Pharma and medical Professionals, healthcare Professionals and leading specialists involved in the field of Pharmaceutical Sciences.

HTML FULL TEXT    PDF 

Design and Evaluation of Medicated Derma Sticks of Azadirachta indica For Antimicrobial Activity

 

M.G.K. Murthy*, K. Purushotham Rao

H.K.E. Society’s College of Pharmacy, M.R. Medical College, Gulbarga-585 105, Karnataka, India

Abstract

Topical skin infections commonly occur in person in worldwide level. Plants reported to possess activity or used in traditional systems of medicine for prevention and treatment of skin disorders. Hence, present study was aimed to design and evaluate medicated sticks of Azadirachta indicaextractwhich is very well known for the antibacterial and antifungal activity. The petroleum ether and ethanol (70%) extracts were prepared. Medicated derma sticks of Azadirachta indicaextract were prepared by heating and congealing and evaluated for thickness, length and weight. The medicated derma sticks of Azadirachta indica were screened for antimicrobial activity against S. aureus, E. coli and C. albicans, and further stability studies was performed. The prepared medicated derma sticks ofAzadirachta indica obtained were of uniform length, thickness and weight respectively. The zones of inhibition of medicated derma sticks ofAzadirachta indica against all the microorganisms were nearer to pure drugs. The stability study of medicated derma sticks ofAzadirachta indica exhibited that the formulations were safe to use in tropical application.

HTML FULL TEXT    PDF    XML 

Formulation and Evaluation of Besifloxacin Loaded In Situ Gel For Ophthalmic Delivery

 

Shivani Kala, Prachi Gurudiwan*, Divya Juyal

Himalayan Institute of Pharmacy and Research Atakfarm, Dehradun, Uttarakhand, India

Abstract

The aim of study was to develop in situ gel of Besifloxacin by using blend polymer of sodium alginate, ethylcellulose and Xanthan gum to increase the pre-corneal residence time and better bioavailability of drug. In situ ocular gels of Besifloxacin (F1 to F6) were prepared by using polymers xanthan gum, Ethyl cellulose and sodium alginate in different ratio. The formulations F1 to F6 were evaluated for Clarity, visual appearance, pH, gelling capacity, drug content, assessment of drug release and ocular irritancy. The F1 to F6 were transparent and clear, and possessed a satisfactory gelling capacity. The drug content capacity for F1 to F6 ranged between 96.24% to 98.63%. The in vitro releases of drug from in situ ocular gel demonstrated that F3 (98.67%) has maximum drug release for 8 hrs compared to other formulations, and showed sustained release. The ocular irritancy study of F3 formulation showed non-irritant and safe to use. The studies suggested that prepared in situ ophthalmic gel of Besifloxacin will be an alternative for conventional eye drops and valuable alternative to counter the precorneal loss.       

HTML FULL TEXT    PDF    XML 

Chemical Constituents From the Roots of Oenothera biennis L.

 

Shahnaz Sultana1,2, Mohammed Ali1*, Showkat Rasool Mir1

1Phytochemistry Research Laboratory, Faculty of Pharmacy, Jamia Hamdard, New Delhi, 110 062, India

2Present address: College of Pharmacy, Jazan University, Jazan, Saudi Arabia

Abstract

Oenothera biennis L. (Onagraceae), is a biennial herb, native to eastern and central North America. It is cultivated in temperate regions of the world and in Indian gardens. The plant parts are used to treat gastro-intestinal disorders, eczema, whooping cough, asthma, blood disorders, laziness, obesity, piles and boils. The dried root powder was exhaustively extracted with methanol and the extract concentrated to yield a dark brown viscous mass. It was dissolved in small quantity of methanol and adsorbed onto silica gel (60 - 120 mesh) for preparation of a slurry. The air dried slurry was subjected to chromatography over silica gel column packed in petroleum ether. The column was eluted successively with petroleum ether,   chloroform  and methanol in order of increasing polarity to  isolate the new phytoconstituents characterized as 3,11,15-trimethyl-14β-hydroxy-n-hexadeca-7-en-4,18-olide (phyt-7-enyl-14β-ol 4β,18-olide, 2), 3-methyl-6α,8β-dihydroxy-7-carboxylic acid tetralin-1,9β-olide (3), 3,7-dimethyl-11-hydroxymethylene dodec-3α,6α-diol- 11-enyl  2′,3′,6′-benzene triol (4), 1,9,10-trimethoxy-3,11-dihydroxy-13-(18,19-dihydroxyprenyl)-anthracene (5) and  α-D-glucopyranosyl–(4→1′)-α-D-glucopyranosyl -6′-cetoleate (6) along with benzoic acid (1). The structures of all the isolated phytoconstituents have been established on the basis of spectral data analysis and chemical reactions.

HTML FULL TEXT    PDF    XML 

Regeneration of Aegle marmelos (l.) Through Enhanced Axillary Branching from Cotyledenory Node

 

Asha Gupta1*, Tessy Thomas1, Shagufta Khan2 

1Department of Botany, Govt. P.G. College, Bhopal (MP)- 462022, India

3GrowTips Biotech, Saket Nagar, Bhopal (MP)- 462024, India

Abstract

A complete protocol is standardized for in vitro micropropogation of Aegle marmelos for the first time using cotyledonary nodes derived through invitro raised seedlings. Higher percentage of direct multiple shoots were regenerated from cotyledonary nodal segments through forced axillary branching. The cotyledonary nodes of invitro raised seedlings were used as explants for shoot formation on MS Medium supplemented with Cytokinins (BAP) and Auxins (NAA), either alone or in combinations. Maximum (80%) shoots having shoot length of 2-3 cm were achieved on MS medium fortified with BAP (1.0 mg/l) and NAA (0.5mg/l). By repeating sub culturing of the cotyledonary node on shoot multiplication medium followed by shoot elongation medium after each harvest of the newly formed shoots. Thus, from a single cotyledonary node, about 20-25 shoots were obtained. Shoots formed in vitro were best rooted on MS medium supplemented with 1.0-2.0 mg/l Napthalene acetic acid(NAA).

HTML FULL TEXT    PDF    XML 

Antioxidant, Anti-alzheimer and Anti-parkinson activity of Artemisia nilagirica Leaves with Flowering Tops

 

Pradeep Pal1*, A.K. Ghosh2

1Mahakal Institute of Pharmaceutical Studies, Ujjain (M.P.)-456664, India

2IFTM University, Muradabad (U.P.)- 244102, India

Abstract

Presently peoples are loaded with stress and leads to various neurodegenerative disorders like anxiety, Alzheimer’s disease and Parkinson’s disease. The present study was aimed to investigate the antioxidant, anti-alzheimer and anti-parkinson activity of Artemisia nilagirica leaves with flowering tops extracts. The ethanol and aqueous extracts of Artemisia nilagirica leaves with flowering tops were processed for evaluation of in vitro antioxidant activity namely hydrogen-donating activity, superoxide scavenging activity, total polyphenol content, total flavonol content and reducing power assay. The object recognition and Y-Maze test were used to evaluate the anti-alzheimer`s activity of extract. The different parameters like catalepsy (bar test), locomotor activity (actophotometer test), and muscle activity (rotarod test) were measured in all animals for anti-parkinson activity. The findings of in vitro antioxidant study demonstrated that ethanol extract has maximum antioxidant properties compared to aqueous extract. Hence the ethanol extract of Artemisia nilagirica was selected for screening of anti-alzheimer and anti-parkinson activity. The administration of ethanol extract of Artemisia nilgirica exhibited significant anti-alzheimer and anti-parkinson activity. The findings of anti-alzheimer and anti-parkinson activity ethanol extract of Artemisia nilagirica demonstrated that this plant have neuroprotective properties.

HTML FULL TEXT    PDF    XML 

Evaluation of Ciprofloxacin Floating-Bioadhesive Tablet Formulated with Okra Gum as Multifunctional Polymer

 

*Alalor CA1, Uhumwangho MU2, Iwuagwu MA2

1Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Delta State University, Abraka, 320001, Nigeria2Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, University of Benin, Benin City, 300001,    Nigeria

Abstract

Floating drug delivery systems and bioadhesive drug delivery systems are gastroretentive systems for increasing gastric residence time to obtain improved drug bioavailability. This study was to evaluate the floating and bioadhesive characteristics of Ciprofloxacin tablets formulated with Abelmoschus esculentus gum (okra gum).Okra gum was extracted and granules were prepared using the extracted Okra gum as well as sodium alginate and HPMC at concentrations of 2.5, 5 and 10 % w/w. Ciprofloxacin floating bioadhesive (CFB) tablets were evaluated for hardness, friability, in vitro buoyancy test, ex vivo bioadhesion test and drug release profiles. The floating lag time (FLT) and total floating time (TFT) for CFB tablets formulated with 10% w/w okra gum were 5.7 minutes and 8 hours respectively while the bioadhesive force was 1.324 N. Formulations of ciprofloxacin tablets containing admixtures of okra gum and sodium alginate or HPMC resulted in significant decrease (p < 0.05) in the floating lag times (≤ 3.1 minutes) and significant increase (p < 0.05) in total floating times (> 12 h). The bioadhesive force for CFB tablets containing admixtures of okra gum and sodium alginate or HPMC gave higher values in the range of 1.766 – 2.207 N. The in vitro release profiles for CFB tablets formulated with okra gum alone did not show sustained release below 10 % w/w. Batches FB10 and FB11 containing admixtures showed sustained release with maximum release of 86% at maximum time of 9 h. The dissolution profiles of tablets from batches F10 and F11 compared favourably with the profile for the commercial brand of floating ciprofloxacin tablet, MF. From the study Okra gum has shown to possess good floating and bioadhesive properties and may be utilized in the formulation gastro-retentive dosage form of ciprofloxacin which can possibly be harnessed as a targeted site-specific delivery system in the eradication of Helicobacter pylori in gastric ulcer disease as well as in the treatment of Salmonella typhi induced enteric fever.

HTML FULL TEXT    PDF    XML