Chemical Constituents From the Roots of Oenothera biennis L.


Shahnaz Sultana1,2, Mohammed Ali1*, Showkat Rasool Mir1

1Phytochemistry Research Laboratory, Faculty of Pharmacy, Jamia Hamdard, New Delhi, 110 062, India

2Present address: College of Pharmacy, Jazan University, Jazan, Saudi Arabia


Oenothera biennis L. (Onagraceae), is a biennial herb, native to eastern and central North America. It is cultivated in temperate regions of the world and in Indian gardens. The plant parts are used to treat gastro-intestinal disorders, eczema, whooping cough, asthma, blood disorders, laziness, obesity, piles and boils. The dried root powder was exhaustively extracted with methanol and the extract concentrated to yield a dark brown viscous mass. It was dissolved in small quantity of methanol and adsorbed onto silica gel (60 - 120 mesh) for preparation of a slurry. The air dried slurry was subjected to chromatography over silica gel column packed in petroleum ether. The column was eluted successively with petroleum ether,   chloroform  and methanol in order of increasing polarity to  isolate the new phytoconstituents characterized as 3,11,15-trimethyl-14β-hydroxy-n-hexadeca-7-en-4,18-olide (phyt-7-enyl-14β-ol 4β,18-olide, 2), 3-methyl-6α,8β-dihydroxy-7-carboxylic acid tetralin-1,9β-olide (3), 3,7-dimethyl-11-hydroxymethylene dodec-3α,6α-diol- 11-enyl  2′,3′,6′-benzene triol (4), 1,9,10-trimethoxy-3,11-dihydroxy-13-(18,19-dihydroxyprenyl)-anthracene (5) and  α-D-glucopyranosyl–(4→1′)-α-D-glucopyranosyl -6′-cetoleate (6) along with benzoic acid (1). The structures of all the isolated phytoconstituents have been established on the basis of spectral data analysis and chemical reactions.


Regeneration of Aegle marmelos (l.) Through Enhanced Axillary Branching from Cotyledenory Node


Asha Gupta1*, Tessy Thomas1, Shagufta Khan2 

1Department of Botany, Govt. P.G. College, Bhopal (MP)- 462022, India

3GrowTips Biotech, Saket Nagar, Bhopal (MP)- 462024, India


A complete protocol is standardized for in vitro micropropogation of Aegle marmelos for the first time using cotyledonary nodes derived through invitro raised seedlings. Higher percentage of direct multiple shoots were regenerated from cotyledonary nodal segments through forced axillary branching. The cotyledonary nodes of invitro raised seedlings were used as explants for shoot formation on MS Medium supplemented with Cytokinins (BAP) and Auxins (NAA), either alone or in combinations. Maximum (80%) shoots having shoot length of 2-3 cm were achieved on MS medium fortified with BAP (1.0 mg/l) and NAA (0.5mg/l). By repeating sub culturing of the cotyledonary node on shoot multiplication medium followed by shoot elongation medium after each harvest of the newly formed shoots. Thus, from a single cotyledonary node, about 20-25 shoots were obtained. Shoots formed in vitro were best rooted on MS medium supplemented with 1.0-2.0 mg/l Napthalene acetic acid(NAA).


Antioxidant, Anti-alzheimer and Anti-parkinson activity of Artemisia nilagirica Leaves with Flowering Tops


Pradeep Pal1*, A.K. Ghosh2

1Mahakal Institute of Pharmaceutical Studies, Ujjain (M.P.)-456664, India

2IFTM University, Muradabad (U.P.)- 244102, India


Presently peoples are loaded with stress and leads to various neurodegenerative disorders like anxiety, Alzheimer’s disease and Parkinson’s disease. The present study was aimed to investigate the antioxidant, anti-alzheimer and anti-parkinson activity of Artemisia nilagirica leaves with flowering tops extracts. The ethanol and aqueous extracts of Artemisia nilagirica leaves with flowering tops were processed for evaluation of in vitro antioxidant activity namely hydrogen-donating activity, superoxide scavenging activity, total polyphenol content, total flavonol content and reducing power assay. The object recognition and Y-Maze test were used to evaluate the anti-alzheimer`s activity of extract. The different parameters like catalepsy (bar test), locomotor activity (actophotometer test), and muscle activity (rotarod test) were measured in all animals for anti-parkinson activity. The findings of in vitro antioxidant study demonstrated that ethanol extract has maximum antioxidant properties compared to aqueous extract. Hence the ethanol extract of Artemisia nilagirica was selected for screening of anti-alzheimer and anti-parkinson activity. The administration of ethanol extract of Artemisia nilgirica exhibited significant anti-alzheimer and anti-parkinson activity. The findings of anti-alzheimer and anti-parkinson activity ethanol extract of Artemisia nilagirica demonstrated that this plant have neuroprotective properties.


Evaluation of Ciprofloxacin Floating-Bioadhesive Tablet Formulated with Okra Gum as Multifunctional Polymer


*Alalor CA1, Uhumwangho MU2, Iwuagwu MA2

1Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Delta State University, Abraka, 320001, Nigeria2Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, University of Benin, Benin City, 300001,    Nigeria


Floating drug delivery systems and bioadhesive drug delivery systems are gastroretentive systems for increasing gastric residence time to obtain improved drug bioavailability. This study was to evaluate the floating and bioadhesive characteristics of Ciprofloxacin tablets formulated with Abelmoschus esculentus gum (okra gum).Okra gum was extracted and granules were prepared using the extracted Okra gum as well as sodium alginate and HPMC at concentrations of 2.5, 5 and 10 % w/w. Ciprofloxacin floating bioadhesive (CFB) tablets were evaluated for hardness, friability, in vitro buoyancy test, ex vivo bioadhesion test and drug release profiles. The floating lag time (FLT) and total floating time (TFT) for CFB tablets formulated with 10% w/w okra gum were 5.7 minutes and 8 hours respectively while the bioadhesive force was 1.324 N. Formulations of ciprofloxacin tablets containing admixtures of okra gum and sodium alginate or HPMC resulted in significant decrease (p < 0.05) in the floating lag times (≤ 3.1 minutes) and significant increase (p < 0.05) in total floating times (> 12 h). The bioadhesive force for CFB tablets containing admixtures of okra gum and sodium alginate or HPMC gave higher values in the range of 1.766 – 2.207 N. The in vitro release profiles for CFB tablets formulated with okra gum alone did not show sustained release below 10 % w/w. Batches FB10 and FB11 containing admixtures showed sustained release with maximum release of 86% at maximum time of 9 h. The dissolution profiles of tablets from batches F10 and F11 compared favourably with the profile for the commercial brand of floating ciprofloxacin tablet, MF. From the study Okra gum has shown to possess good floating and bioadhesive properties and may be utilized in the formulation gastro-retentive dosage form of ciprofloxacin which can possibly be harnessed as a targeted site-specific delivery system in the eradication of Helicobacter pylori in gastric ulcer disease as well as in the treatment of Salmonella typhi induced enteric fever.