Manish Negi*, Vikash Kumar Shukla, T. S. Easwari
IIIMT College of Medical Sciences, Mawana Road, Meerut-250001, (U.P.), India
Floating microspheres of Ofloxacin were prepared by emulsion cross linking method in which form two phase solution. A total of six formulations were prepared i.e. F1, F2, F3, F4, F5 and F6. Microsphere were evaluated for various rheological properties like bulk density, tapped density, Hausner’s ratio, angle of repose, shows satisfactory results. The prepared microsphere were then evaluated for various physical tests like particle size, drug content, drug entrapment efficiency, swelling index and buoyancy test by using standard procedures. The particle size of all the formulations was ranged between 158.6±0.62 and 205.1±0.42 μm. The drug content and entrapment efficiency was ranged between 18.4±0.48 to 31.1±1.02% and 53.0±1.48 to 76.6±0.94%, respectively. The swelling index and buoyancy were ranged from 79.9±1.42 to 110.6±1.37 and 94.2±0.29 to 121.9±0.19, respectively. The prepared microspheres exhibited good spherical geometry with smooth surface as evidence by scanning electron microscopy. The in vitro release studies exhibited that F3 displayed better sustained effect over a period of 16 hours.HTML FULL TEXT PDF XML
Prasanna Purohit1,2, Ritu Thakur Bais2, Pratibha Sing2, Shagufta khan1
1Department of Botany, Sarojini Naidu Govt. Girls P.G.Autonomous College, Shivaji Nagar Bhopal (MP),India
2Grow Tips Biotech, Bhopal (M.P), India
Hemidesmus indicus belongs to the family Asclepiadaceae, and it is exceptionally demanded by traditional healers for the ailment of various diseases. The over exploitation of Hemidesmus indicus placed it under the endangered medicinal plant, and it required to develop in vitro culture for conservation and maintain the sustainable demand of plant. In the present study, we planned to develop rapid and efficient protocol for large-scale propagation of Hemidesmus indicus through in vitro culture. Cotyledons from in vitro germinated seeds were used as initial explants, inoculated in MS medium supplemented with various cytokinins, BAP(0.5-2.0 mg/l) and kn (0.5-1.0 mg/l)) in combination with auxin, NAA (0.5-1.0 mg/l) and IAA(0.1-0.5 mg/l). The optimal response of shoot initiation (80%) with average number of shoots 6.8 ± 0.10 ( mean length 6.4 ± 0.13) was observed in the medium (I-9) containing 2.0 mg/l BAP and 0.5 mg/l NAA within 4 week. The maximum shoot multiplication (84%) with average number of shoots 24.9 ± 0.15, was observed on MS medium M5 containing BAP (1.0 mg/l) in combination with Kn (0.5 mg/l). regenerated shoots were excised aseptically and implanted on MS half and full strength medium fortified with various concentrations of IBA, IAA and NAA for root formation. Full strength MS medium (R9) having 0.5 mg/l IAA was found better with 60% root formation after 16-18 days. The rooted plantlets were successfully acclimatized in pots containing sterilized soil and sand mixture (3:1) with 95% survival rate in the field conditions.HTML FULL TEXT PDF XML
Jiyauddin K.1*, Zulhabri O.2, Aishah U. A. M.2, Rasha S.1, Hamid K.1, M. Qamar1, S. Budiasih1, Jawad A.1, Samer A. D.1, M Kaleemullah1, Rasny M. R.1, Gamal O. E.3, Eddy Y.1, Fadli A.1 and Junainah A. H.1,2
1School of Pharmacy, Management & Science University, 40100 Shah Alam, Selangor Darul Ehsan, Malaysia.
2Faculty of Health and Life Sciences, Management & Science University, 40100 Shah Alam, Selangor Darul Ehsan, Malaysia.
3Unaizah College of Pharmacy, Qassim University, Qassim, Kingdom of Saudi Arabia.
The antioxidant and antimicrobial activities have been performed using methanol fruit extract of Artocarpus altilis. Results of the phytochemical screening revealed that methanolic extract of A. altilis fruits contain flavonoids, phenols, steroids and glycosides. The investigation on these constituents was done using the phytochemical screening with modification. The soxhlet apparatus was employeded to extract the dried plant using methanol as a solvent. It was observed that the extract has characteristics of antioxidant using DPPH assay. The antibacterial and antifungal potential of methanolic fruit extract were studied against Staphylococcus aureus, Klebsiella pneumonia, Salmonella spp, Penicillium notatum, Aspergillus niger and Candida albicans. This test was performed by using disc-diffusion method with different concentrations of fruit extract. It was found that one bacterial species (i.e. Staphylococcus aureus) showed inhibition zone with maximum (zone of inhibitionÃ¢â€°Ë†15mm) using 150 mg/ml of fruit extract. Methanolic extracts of A. altilis had no effect on growth of the remaining microorganisms. The phytoconstituents and characteristics of microorganisms might be responsible for the antimicrobial activity of the extract. Further purifications of secondary metabolites and structural studies may lead to the isolation of the active compounds.HTML FULL TEXT PDF XML
Modern Sciences and Arts University, 26 July Mehwar Road intersection with Wahat Road, 6 October City, Egypt, Postal code: 11787
It has been evaluated that approximately 50% of all drug molecules marketed as medicinal products are administered in a form of salts as they offer many benefits for the pharmaceutical drugs. Salt formation is a relatively simple and powerful pre-formation technique that can result in significant improvement of drug’s physicochemical properties. Following the formation of candidate salt forms, the optimal form is chosen for further development. Salt selection is a rational and step-wise process, in which salts are analysed with regard to particular properties and the most suitable forms are examined further to ensure an optimal choice. Although the effects of salt formation may be highly beneficial to the drug’s performance, there are a number of properties that may deteriorate as a result of the drug being formulated as a salt.HTML FULL TEXT PDF XML
Chin Mee Wong; Jing Jing Ling
Research & Development Division, Malaysian Pepper Board, Lot 1115, Jln. Utama, Pending Industrial Area, 93450 Kuching, Sarawak, Malaysia.
Piper nigrum L. is a perennial climbing vine and its dried; ground berries make one of the most common spices in worldwide cuisine. Besides its extensive culinary uses, black pepper is commonly mixed in home remedies to heal wound and cut. Hence, the current research study was aimed at discovering potential wound healing properties of black pepper berries. A preliminary study was also carried out to determine some major phytochemicals in black pepper berries extracted with different solvents. In vitro cell-based assays were adopted for the observation of wound healing activity. The present study demonstrated that the extracts of the black berries of Piper nigrum L. (0.32-1.0 µg/ml) encouraged cell migration activity, notwithstanding existence of contrasting activity as the concentration increased. The observed wound healing activity was most probably due to the presence of phytochemicals, viz.; flavonoids and triterpenes.HTML FULL TEXT PDF XML
Rajendra Jangde*, Deependra Singh
University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur, C.G., India (492010)
The aim of the present study was tochecked the compatibility between drug: lipid and drug: excipients. Ellagic acid was selected as model drug while phospholipid and Cholesterol were considered excipients. The study was performed by Fourier transform infrared spectroscopy (FTIR), Diffraction scanning calorimetric (DSC) and High performance liquid chromatography (HPLC). The spectrophotometric graphs revealed that there was no significant changes in the position of functional groups of ellagic acid, phospholipid and Cholesterol (O-H, C=O, C-H str.) in pure drugs and excipients with respect to their physical mixtures. Further the samples werecharacterized by using DSC and HPLC methods and found no any interaction between drug excipients. Combination of synthetic herbal drug and pharmaceutical excipients are compatible with each other and shows no interaction. Thus this suitable combination can be employed for the successful development of novel liposomal carrier system.HTML FULL TEXT PDF XML