Preliminary Phyochemical Analysis and Characterization of Flavonoid Moiety from Vitex negundo Leaves Origin in Madhya Pradesh State by HPLC Study 

Firdous Ahmad Dar1*, Kirti Jain2, Bharti Jain3,  Madhuri Modak1

1Government Motilal Vigyan Mahavidyalaya,  Jahangirabad, Bhopal-462004, Madhya Pradesh, India

2Govt. Science and Commerce College Benazir, Jahangirabad, Bhopal-462008, Madhya Pradesh, India

3Sarojini Naidu Govt Girls PG Autonomous College, Bhopal (M.P.)-462016, India

Received: 20-Jul-2017 , Accepted: 25-Oct-2017

Keywords: Vitex negundo, Phytochemical analysis, HPLC, Flavonoids

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Firdous Ahmad Dar, Kirti Jain, Bharti Jain,  Madhuri Modak. Preliminary Phyochemical Analysis and Characterization of Flavonoid Moiety from Vitex negundo Leaves Origin in Madhya Pradesh State by HPLC Study.UKJPB. 2017;5(5), 27-32. 

Abstract

In the present study leaves of an important medicinal plant Vitex negundo was subjected to a phytochemical screening, flavonoid content and characterization of flavonoids moiety of Vitex negundo leaves extracts. The petroleum ether, chloroform, ethanol, methanol and aqueous extracts were prepared, and further screened for preliminary phytochemical test. The quantification of quercetin was investigated in ethanol extract by HPLC study. Preliminary phytochemical revealed the presence of flavonoids and phenolic compounds in ethanol, methanol and aqueous extracts of leaves of Vitex negundo. The ethanol, methanol and aqueous extracts investigated for antioxidant activity, and ethanol extract indicates the presence of highest quantity of flavonoids and polyphenol compared to other extracts. The HPLC chromatogram confirmed the flavonoids moiety was quercetin in ethanol extract, and the yield of quercetin was 42.18 ng/ml