Effect of Extraction Solvents on Bioactive Compounds and Antimicrobial Activities of Two Varieties of Garcinia kola (heckel)OBOWO 02 (soft and less bitter) and OBOWO 03 (Hard and Very Bitter)
Okwulehie I. C*., Alozie V. C., Ikechukwu G. C., Nwokeocha O. W.
Michael Okpara University of Agriculture, Umudike, PMB-7267, Umuahia, Abia State, Nigeria
Received: 30-Nov-2017 , Accepted: 21-Jan-2018
Keywords: Garcinia kola, Extraction solvents, Bioactive composition, Anti-microbial
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Okwulehie IC, Alozie VC, Ikechukwu GC, Nwokeocha OW. Effect of Extraction Solvents on Bioactive Compounds and Antimicrobial Activities of Two Varieties of Garcinia kola (heckel)OBOWO 02 (soft and less bitter) and OBOWO 03 (Hard and Very Bitter). UKJPB. 2017; 5(6): 20-25.
The aim of this study was to compare the effectiveness of the extracts obtained using the different solvents - water, acetone, methanol and ethanol on the phytochemicals and antimicrobial activity of Garcinia kola (Obowo 20 and 03). The following 4 bacterial isolates were used for the investigation of the antimicrobialactivities and and the minimum inhibitory concentration (MIC) of the extracts. The phytochemical analysis of each of the extract indicated the presence of tannin, saponin, flavonoid, Hydrogen cyanide (HCN), alkaloid, carotenoid, anthocyanin and phenol. The extracts exhibited significant inhibitory action against S. aureus, S. typhi, P. aeruginosaand E. coli. The result revealed that methanol extract exerted the highest significant activities (P> 0.05) against all the tested organisms at the various treatment regimes with S. aureus having a wider zone of inhibition followed by E. coli, P. aeruginosa and S. typhiwith the lowest inhibitory zones. The MIC of the methanol extract against the organisms was 12.5, 12.5, 25 and 50 mg/ml respectively. The aqueous extract showed the least significant activity against S. aureus, E. coli, P. aeruginosa and S. typhi with MICs of 25, 25, 25 and 50mg/ml respectively.