Alterations in Zeta Potential and Osmotic Fragility of Red Blood Cells in Hyperglycemic Conditions

Swati S. Gaikwad*, Megha N. Karemore, Jasmine G. Avari

Department of Pharmaceutical Sciences, R. T. M. Nagpur University, Mahatma Jyotiba Fuley Educational Campus, Amravati Road, Nagpur, 440033, Maharashtra, India

Received: 04-Apr-2018 , Accepted: 01-Jun-2018

Keywords: Diabetes Mellitus, Gestational Diabetes Mellitus (GDM), Red Blood Cells (RBCs), Zeta Potential, Osmotic Fragility, Lipid peroxidation, Hyperglycemia



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Swati S. Gaikwad, Megha N. Karemore, Jasmine G. Avari. Alterations in Zeta Potential and Osmotic Fragility of Red Blood Cells in Hyperglycemic Conditions. UKJPB. 2018; 6(3): 25-30.


The zeta potential is an electrokinetic property of red blood cells surface and in different disease conditions this property of the erythrocytes varies. The objective of the present study was to evaluate the alteration in zeta potential, lipid peroxidation and osmotic fragility of erythrocytes in hyperglycemic conditions.  The zeta potential of the RBCs was measured using Zeta meter System 4.0. Lipid peroxidation, an indicator of tissue injury induced by reactive oxygen species was measured by the thiobarbituric acid assay (TBA). The percent haemolysis in terms of osmotic fragility was determined using UV-Vis spectrophotometer (JASCO).The mean erythrocytic ZP of the control group was found to be 22.13±0.2789 mV whereas, erythrocytic ZP for diabetes mellitus patients was found to be 8.559±0.4864 mV. Similarly, when erythrocytic ZP of control pregnant women was measured, and it was found to be 21.07±0.3393 mV which were slightly lower than a control group. Mean ZP of GDM patients was found to be 10.12±0.2294 mV which was significantly less than both control group and pregnant control group. Variations in zeta potential values were accompanied by increased osmotic fragility of RBCs. It was also observed from determination of lipid peroxidation of erythrocytes, that there was formation of higher concentration of malondialdehyde with the erythrocytes of hyperglycemic patients compared to control group.The findings suggest that the zeta potential value of erythrocytes can act as a key indicator for demonstration of increased oxidative stress.