Bioassay-Guided Fractionation of Wound Healing Active Compounds From Piper nigrum L. Berries Extract in Malaysia

Chin Mee Wong1*, Jing Jing Ling1, Dr. Paul M. Neilsen2,3, Dr. Siaw San Hwang3

1Department of Research and Development, Malaysian Pepper Board, Lot 1115,  Jalan Utama, Pending Industrial Area, 93450 Kuching, Sarawak, Malaysia

2School of Health, Medical and Applied Sciences, CQUniversity, Bruce Highway, 4702, North Rockhampton, QLD, Australia

3Faculty of Engineering, Computing and Science, Swinburne University of Technology (Sarawak Campus), Jalan Simpang Tiga, 93350 Kuching, Sarawak, Malaysia

Received: 20-Apr-2018 , Accepted: 08-Aug-2018

Keywords: Piper nigrum L., Piperine, Scratch assay, Keratinocyte, Wound healing activity

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How to Cite this Article

Wong CM, Ling JJ, Neilsen PM, Hwang SS. Bioassay-Guided Fractionation of Wound Healing Active Compounds From Piper nigrum L. Berries Extract in Malaysia. UKJPB. 2018; 6(4): 19-27.

Abstract

The study aimed to further evaluate the wound healing property of P. nigrum L. using bioassay-guided fractionation method. The ethanolic extract of Piper nigrum L. was fractionated in two stages using column chromatography and preparative reversed phase C18 HPLC, respectively. Significant wound healing properties screened using the scratch wound assay was observed through the cell migration assay in fraction number three (PNE3) out of the 14 fractions, exhibiting 36.7% and 43.8% closure of wound gap within 20 hours at concentration of 0.3 and 1.0 µg/mL, respectively. Sub-fractions which were further fractionated from PNE3 showed comparatively reduced wound healing activity using the same bioassay. The sub-fractions were also compared to Piperine, a major component of P. nigrum L. and the results were comparable. This experimental study revealed that Piperine works together with other compounds in P. nigrum L. to improve wound healing as claimed by those home remedy.