Synthesis of 2,2`-Bis-7-Substituted-[1,3,4]Thiadiazolo-[2,3-B]Quinazolin-5-one and Screened for Analgesic Activity

 

Dipu Kakoty*, B. Shivakumar, Kamal Nayan

Drug Testing Laboratory (AYUSH), Govt. Ayurvedic College, Jalikbari, Guwahati-781014, Assam

Abstract

A series of 2,2`-bis-7-substituted-[1,3,4]thiadiazolo-[2,3-b]quinazolin-5-one were synthesized by action of 3-amino-2-mercaptoquinazolinone-4(3H)-one. By the analytical and spectral properties of IR, H1NMR and Mass, newly synthesized compounds are characterized. The present new series of compounds have been subjected to the analgesic (in vivo) activities by standard methods. Results of the activities reveals that, none of the compounds exhibited good to significant analgesic activities. 

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Evaluation of the Chelating Efficacy of Aquilaria malaccensis and Aristolochia longa Against Biochemical Alterations Induced by Lead Bioaccumulation in Rats

 

Derouiche Samir*, Zeghib Khaoula

Department of Cellular and Molecular Biology, Faculty of natural science and life, University of Echahid Hamma Lakhdar-Eloued , El-oued 39000, El-oued, Algeria

Abstract

The current study was evaluated the chelating effect of Aquilaria malaccensis and Aristolochia longa against lead induced biochemical alterations and bioaccumulation of lead in tissues of rats. 25 adult female Wistar albino rats, equally divided into control and four treated groups, received either lead, lead + A. malaccensis, lead + A. longa and lead + A. malaccensis + A. longa  lead (100 mg/kg b.w) as Pb(C2H3O2)2 added in their drinking water for 75 days. A. malaccensis and A. longa (at a dose 1% of diet) were added to the feed during the last 15 days of lead exposed in the animals. Result showed that in lead-intoxicated rats, an increase of lead accumulation in serum, bone and liver of rats. Results also revealed that lead affected metabolic system by increasing blood glucose and serum urea concentrations and decreasing serum calcium concentration. Treatment with A. malaccensis and A. longa alone or combined significantly reduced the adverse effects related to most of serum and tissue lead accumulation and restored biochemical parameters alterations in animals treated with lead. The present study shows that A. malaccensis and A. longa are an effective chelating agents for the removal of lead and it has proved efficient in restoring both the biochemical alteration and toxicity after a sub lethal exposure of lead acetate in rats.

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Comparative Analysis of Piperine in Wild Plant and Callus of Piper longum by HPLC Method

 

Sifat Siddique1*, Tessay Thomas2, Shagufta Khan3

1Chandra Shekhar Azad, Govt. P. G. Nodal College, Sehore (M.P.)- 466001, India

2Govt. P. G. College, Guna (M.P.)- 473001, India

3Grow Tips Biotech, Saket Nagar, Bhopal (MP)- 462024, India

Abstract

Piperine is chief bio-molecular active compound of Piper longum, exhibited various pharmacological activities.  In addition it improves the bioavailability of other nutritive substances. The aim of the study was to induce callus from Piper longum, and compared the quantification of piperine in callus and wild grown Piper longum.The callus were produced by transferring the sterile leaves on MS medium containing different concentration of cytokinins like 0.5 mg/l BAP (6-benzylaminopurine), 1.0 mg/l KN (kinetin) with auxins like 0.5 - 2.0 mg/l 2,4-D (2 4-dichlorophenoxyacetic acid). The petroleum ether, ethanol and aqueous extracts of Piper longum plant and callus were prepared. The content of piperine in plant extract and callus extract were performed by HPLC. The phytochemical study uncovered the nearness of different secondary metabolites in various extract of plant. The HPLC chromatogram displayed that content of piperine present in callus extract was higher compared to field grown plants.

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Evaluation of Antimicrobial Activities of Sinapis alba and Brassica nigra Leaves Against Selected Microorganisms 

 

Hawaz Weldu1*,Abel Mehari2, Lia Alem3

 1Haz-haz Zonal Referral Hospital Asmara-P.O.Box-9098, Eritrea

2Eritrea Pharmacological, product and supplies, Asmara-P.O.Box-1689, Eritrea

3National TB reference Laboratory, National Health Laboratory, Asmara-P.O. Box-1686, Eritrea

Abstract

Many traditional practitioners in developing countries use the herbaceous plant to treat a different type of microbial infection. Eritrea is one of the developing countries where most of their communities are dependent on herbal medicines for the treatment of infectious disease. However, this malpractice follows incorrect dosage, administration, formulation, frequency and other non-scientific methods with the inevitable negative effect of the practice which makes it inconvenient for the clients who seek treatment. Therefore the current study was carried out to get a scientific evidence of antimicrobial activity of two selected important herbal plants. Active part from leaves of Sinapis alba and Brassica nigra were extracted by continuous hot extraction (Soxhlet technique), and different concentrations were obtained by ethanol, N-hexane, aqueous and DMSO solvents. Microorganisms (Escherichia Coli and Staphylococcus aureus from bacterial strains and Candida Albicans from fungal strain) were selected for testing antimicrobial activity of the plants. Then the extracted solutions were diffused to selected standard organisms inoculated in Muller Hinton Agar using well diffusion technique. Ethanol extracts of S. Alba of 2500mg/ml dissolved in DMSO concentration against E. coli have shown a significant activity with inhibition zones of 30mm. This plant in the same concentration also had a considerable effect against S. aureus and C. Albcaians with a prompting result of 28mm and 25mm zones of inhibition respectively which is greater than the positive control. Moreover, this plant showed almost an equal activity at 1000mg and 250mg which are 20mm and 13mm respectively for  C. Albicans, 26mm and 23mm for S. aureus and for E. coli 25mm and17mm. N-hexane extracts of the same plant also showed a remarkable activity at concentrations of 1000mg, 250mg and 50mg, where the zones of inhibition against S. aureus were 18mm, 20mm and 25mm respectively. Ethanol-extract of this plant diluted in ethanol also showed activity at the lowest concentration. Generally, both plants extracted using N-hexane and Ethanol extracts gave a remarkable activity against all the selected micro-organisms.

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