Phytochemical Constituents and Antimicrobial Activities of the Essential Oils of Libyan Pituranthos chloranthus on Different Species of Bacteria

 

Yahya Saber E Mansour1*, Nusieba A Mohammed Ibrahim1, Yaroub S Sergiwa2, Krishna Chaitanya Varma Sagi3  

1Department of Pharmacology and Toxicology, Faculty of Pharmacy, Omar Al-Mukhtar University, Al-bayda, Libya

2Department of Microbiology, Faculty of Science, Omar Al-Mukhtar University, Al-bayda, Libya

3Qpathlabs, Visakhapantnam, Andhra Pradesh, India

Abstract

Essential oils (EOs) obtained from fresh and dry aerial parts of Libyan Pituranthos chloranthus were analyzed and isolated by Gas Chromatography/Mass Spectrometry (GC/MS) and hydro-distillation technique. The main constituents of the EOs obtained from fresh herb of P. chloranthus were found to be α-pinene, sabinene, cis-β-ocimene, and myrcene whereas the major components of the EOs derived from dry herb of P. chloranthus were α-phellandrene, Δ-3-carene, and β-phellandrene.Minor changes in the phytochemical compositions of the P. chloranthus EOs obtained from fresh or dry herbs; however, the major constituents were found to be α-pinene, sabinene, cis-β-ocimene, myrcene, α- phellandrene, Δ-3-carene, and β-phellandrene with approximate amounts of p-cymene, limonene, trans-β-ocimene, γ-terpinene, and cis-verbenol. The paper-disc agar diffusion method was used to evaluate the antimicrobial activities of P. chloranthus, and the results showed that the EOs extracted from fresh herb had a significant inhibitory effect against most of the tested bacteria.

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Standardization of an Efficient Protocol for In Vitro Micropropagation of Centella asiatica L. – An Important Medicinal Plant

 

Sifat Siddiqui1, Tessy Thomas2, Shagufta khan3

1Chandra Shekhar Azad, Govt. P. G. Nodal College, Sehore (M.P.)- 466001, India

2Govt. P. G. College, Guna (M.P.)- 473001, India

3Grow Tips Biotech, Saket Nagar, Bhopal (M.P.)- 462024, India

Abstract

The present study was undertaken to evaluate the most suitable and efficient media of low concentration growth regulators for shoot induction and subsequent multiplication in Centella asiatica L. an important and valuable medicinal plant. The best shoot induction (82±2.2%) was found in MS medium supplemented with 0.5 mg/ l BAP + 0.1 mg/ l NAA from axillary meristem explant. Combination of 0.5 mg/ l BAP + 0.5 mg/ l NAA and 0.5 mg/ l BAP + 0.5 mg/ l KN gave the most effective shoot multiplication from initiated meristem cultures. The elongated shoots were rooted in MS medium supplemented with different concentrations of auxins (NAA and IBA). Lower concentration of IBA was more suitable for root induction when compared to NAA. The success of plant tissue culture for in vitro culture of Centella asiatica was encouraged by acclimatization of the plantlets in the field conditions. About 95% plantlets survived under field conditions.

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Synergistic Antidiabetic and Antihyperlipidaemic Activity of Glibenclamide by Integrating Momordica charantia Fruit Juice in Streptozotocin Induced Diabetic Rat

 

Arpit Patidar*, Dr J.S Vaghela, Arijit Chaudhuri, Pahadiya Anitakumari Rambhai

Bhupal Nobles College of Pharmacy, Udaipur-313001, Rajasthan, India

Abstract

Diabetes mellitus is among the most common disorder in developed and developing countries, and the disease is increasing rapidly in most parts of the world. It has been estimated that up to one-third of patients with diabetes mellitus use some form of complementary and alternative medicine. The aim of the present study was to evaluate synergistic antidiabetic and antihyperlipidaemic effects of Momordica charantia fruit juice along with Glibenclamide (lower dose) on glucose tolerance and lipid profile in streptozotocin induced diabetic rat. The Momordica charantia fruit juice (MCFJ) were prepared and different combination of MCFJ and Glibenclamide was evaluated for antidiabetic activity in experimental animals. The administration of MCFJ along with Glibenclamide significantly decreased the blood glucose levels compared to control diabetic rats. The MCFJ along with Glibenclamide significantly lowered the elevated total cholesterol, triglycerides (TGL), total protein and low density lipoprotein (LDL) level while increased the high density lipoprotein (HDL) indicates the antihyperlipidaemic activity. The findings demonstrated the MCFJ enhanced antidiabetic and antihyperlipidaemic activity of Glibenclamide and produced higher protective effect from diabetes.

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Protective Effect of Ethanol Extract of Vanda tessellata Leaves on Methotrexate Induced Hepatotoxicity and Nephrotoxicity in Rats

 

Arijit Chaudhuri, Yuvraj Singh Sarangdevot, Mangilal Choudhary, Arpit Patidar, Pahadiya Anitakumari Rambhai

Bhupal Nobles College of Pharmacy, Udaipur-313001, Rajasthan, India

Abstract

Drugs and chemicals are considered as one of the major causes for hepatotoxicity and renal toxicity, inducing direct damage through multiple pathways including oxidative stress. The present study was aimed to investigate the protective effect of ethanol extract of Vanda tessellata leaves in methotrexate induced hepatotoxicity and nephrotoxicity rats. The ethanol extract of Vanda tessellata leaves were prepared and its protective effect were evaluated for methotrexate induced liver and kidney damaged in rats. The administration of ethanol extract of Vanda tessellata (150 and 300 mg/kg) significant (p<0.05) decrease in serum SGOT, SGPT, ALP, ACP and total bilirubin compared to control group rats treated MTX. Treating with ethanol extract of Vanda tessellatashowed significant decrease (p<0.05) in concentration of serum urea, Creatinine, Uric acid, Total protein and BUN compared to MTX treated groups. The findings of biochemical parameters illustrated that the ethanol extract of Vanda tessellata leaves showed protective properties against MTX induced hepatotoxicity and nephrotoxicity in rats.

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Antiulcer and Antihyperlipidaemic Efficacy of Catharanthus roseus Leaves on pylorus ligation induced Ulcer in Experimental Animals

 

Pahadiya Anitakumari Rambhai*, Dr. S.S. Sisodia, Arijit Chaudhuri, Arpit Patidar

Bhupal Nobles‘ College of Pharmacy, Udaipur-313001, Rajasthan, India

Abstract

The present study was undertaken to scientifically validate the antiulcer and antihyperlipidaemic activity of leaves of Catharanthus roseus against pylorus ligation induced gastric ulcer in experimental animals. The methanol extract of leaves were prepared, and phytochemical analysis of extract were performed. The antiulcer activity as seen by the area of gastric volume, free acidity, total acidity, pH, percentage of ulcer protection and ulcer index were estimated. The antihyperlipidaemic activity with following parameters cholesterol, high density lipoprotein (HDL), Triglycerides (TGL), low density lipoprotein (VLDL), Cholesterol/HDL and low density lipoprotein (LDL) were also evaluated. The phytochemical analysis of methanol extract of leaves of Catharanthus roseus demonstrated the presence of flavonoids, alkaloids, steroids, glycosides and saponin. The extract (125, 250 and 500 mg/kg) and standard drug treated animals significantly decreased the gastric volume, total acidity, free acidity and ulcer index; and  pH of the gastric juice was significantly increased compared to ulcer control group. The extract and standard drug treated rats significantly decreased in TGL, total cholesterol, VLDL, Cholesterol/HDL and LDL, and increased HDL compared to ulcer induced control group rats. The above finding suggests the antiulcer and antihyperlipidaemic activity of Catharanthus roseus extracts. 

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In vitro Callus Cultures for Enhanced Production of Flavonoid (Quercetin) Content, its Analysis and Antibacterial Activity of Nyctanthes arbortristis

 

Ruchita Shrivastava1*, Ajay Kumar Bharadwaj2

1N.M.V. Govt. College, Hoshangabad- 461001, MP, India

2Dept. of Biotechnology, Institute for Excellence in Higher Education, Bhopal- 462016, MP, India

Abstract

Flavonoids have stirred extensive intrigue in light of their potential helpful impact on human wellbeing. Quercetin is a standout amongst the most copious regular flavonoids present in therapeutic plants. The present study was planned to study the in vitro production of flavonoids in callus culture of Nyctanthes arbortristis using different plant growth regulators, and screening of antibacterial activity of ethanol extract of leaves. The callus was produced by transferring the sterile leaves on MS medium containing different concentration of cytokinins like 0.5 mg/l BAP (6-benzylaminopurine), 1.0 mg/l KN (kinetin) with auxins like 0.5 - 2.0 mg/l 2,4-D (2 4-dichlorophenoxyacetic acid). The petroleum ether, ethanol and aqueous extracts of Nyctanthes arbortristis leaves and callus were prepared. The content of quercetin in plant extract and callus extract were performed by HPLC. The antibacterial activity of ethanol extract of leaves were performed by using agar well diffusion and spectroscopy antibacterial activity method. The phytochemical study uncovered the nearness of different secondary metabolites in various extract of plant. The HPLC study confirmed the presence of methoxylated flavonoid quercetin-3,3’-dimethoxy-7-0-rhamnoglucopyranose as a active component in extract. The callus contained higher amount of quercetin compared to fresh leaves. The antibacterial screening results showed that ethanol extract of Nyctanthus arbortristisleaves has been observed an efficient antibacterial effect against different microbes namely S. aureus, E.  coli, S. epidermidis and P. aeruginosa. 

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