Evaluation of Chemoprotective and Anticarcinogenic Activity of Cuscuta reflexa

 

Shweta Mishra1,2­, Jignyasha Amit Rawal2, Ram Kumar Sahu3 

1GRKIST Pharmacy College, Barela, Kukrikheda, Jabalpur (M.P.)-483001, India

2Department of Pharmacy, Pacific University, Udaipur (R.J.)-313001, India

3University College of Pharmacy, Pt. Deendayal Upadhyay Memorial Health Sciences and Ayush University of Chhattisgarh, Raipur (C.G.)-493111, India

Abstract

The plant products are best option for the treatment of cancer due to its lower side effects. In the present study, it was planned to evaluate the chemopreventive and anticarcinogenic activity of Cuscuta reflexa extract in experimental rats. The hydroalcoholic extract was prepared, and acute toxicity, anticarcinogenicity activity and Chemopreventive effect were evaluated. The hydroalcoholic extract was free from toxicity upto dose of 5000 mg/kg body weight. The present investigation showed significant effect of the hydroalcoholic extract (250 and 500 mg/kg) in preventing melanoma tumor by B16F10 cell line in experimental animals. The result of micronucleus assay indicate that hydroalcoholic extract (250 and 500 mg/kg) have preventive potential against cyclophosphamide induced micronucleus formation in experimental animals. The findings suggested that the anticarcinogenicity and chemopreventive activity of Cuscuta reflexa may be due to antioxidant activity.   

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Hepatoprotective Activity of Isolated Flavonoids of Ficus glomerata in Carbon Tetrachloride Induced Hepatotoxicity in Rats

 

Prashant Kalambe*, Hemant Sharma

Faculty of Pharmacy, Sri Satya Sai University of Technology & Medical Sciences, Sehore, (M.P.), India                            

Abstract

The present examination was planned to isolate flavonoids from Ficus glomerata leaves separates, and surveyed their hepatoprotective action against CCl4 instigated liver toxicity in rodents. The petroleum ether, ethanol and aqueous extracts were prepared and screened for phytochemical examination. The in vitro antioxidant activity namely DPPH, Superoxide Scavenging, total polyphenol content and total flavonol content activity of ethanol and aqueous extract were investigated. The distinctive portions were detached from ethanol extracts by utilizing column chromatography. The fractions F5, F7 and F8 were screened for hepatoprotective activity. The phytochemical study demonstrated the flavonoids and polyphenol components present in ethanol and aqueous extract. The findings of in viro antioxidant activity suggested the ethanol extract have higher quantity of polyphenol and flavonoid component compared to aqueous extract. The fractions F5, F7 and F8 shows prominent hepatoprotective activity by altogether diminishing the CCl4 initiated modification in SGOT, SGPT, ALP, ACP, total bilirubin and direct bilirubin in blood. The isolated compound altered the SOD, GPx, CAT and lipid peroxidation level, it indicates the antioxidant activity of isolated components. The findings of study recommend that the flavonoid compound F7 of Ficus glomerata showed higher hepatoprotective activity compared F5 and F8.    

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Phytochemical and Antimicrobial Activity of Ethanol Extract of Madhuca longifolia Flowers

 

Pradeep Pal*, Mughisa Nagori, Dharmendra Makwana, Ajay Sharma

Mahakal Institute of Pharmaceutical Studies, Ujjain, (M.P.)-456664, India

Abstract

The advent of multidrug resistance among pathogenic bacteria is imperiling the worth of antibiotics, which have previously transformed medical sciences. Further, commercial antibiotic drugs caused side effects to body. However, herbal remedies often do not produce any side effects, and safe to be use. Hence the aim of the present study was to perform phytochemical study, and evaluate antimicrobial activity of ethanol extract of Madhuca longifolia against various bacterial and fungal species. The ethanol extract of flower of Madhuca longifolia were prepared and screened for phytochemical study. The different concentration of ethanol extract (50 μg/ml, 100 μg/ml, 150 μg/ml, 200 μg/ml and 250 μg/ml) was evaluated for antimicrobial activity against bacteria and fungi by disc diffusion method. The phytochemical study indicates the presence of alkaloids, glycosides, carbohydrates, flavonoids, tannins and polyphenol in ethanol extract. The extract demonstrated the mild to moderate antibacterial and antifungal activity. The maximum antibacterial activity of extract was found on Staphylococcus aureus, while the highest antifungal activity was found on Aspergillus niger. The present study was concluded that the antimicrobial activity of Madhuca longifolia might be due to presence of flavonoids and polyphenol component.     

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Formulation and Optimization of Ketoprofen Loaded Solid Lipid Nanoparticles Using Central Composite Design

 

Avantika Srivastava*, Sovindra Kumar, Priyanka Kushwaha, Padmanbh Maddheshiya, Malvika Srivastava

Radharaman College of Pharmacy, Ratibad, Bhopal (MP)-462044, India

Abstract

Ketoprofen is belongs to Non-steroidal antiinflammatory drugs (NSAIDs) and characterized by low solubility and bioavailabilty. The present study was planned to explore potential of ketoprofen loaded solid lipid nanoparticles (SLN) as a drug carrier system. Ketoprofen SLN was prepared by solvent injection technique. The formulation was optimized by experimental design considering the concentrations of drug and lipid. The optimized formulation demonstrated reduce particle size, better entrapment efficiency and sustained drug release pattern. The optimized formulation showed sustained release of drug from coating capsules containing lyophilized SLNs with cellulose acetate phthalate in the simulated gastrointestinal fluid. It was concluded that the Ketoprofen loaded SLN based formulation incorporated in capsules was suitable for oral application and give better therapeutic effects compared to conventional dosage form. 

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Isolation and Partial Identification of Bacteria and Fungi from Fermentating Vegetable Garbage of Kitchen

 

Pooja Thapak1*, Neelam Tripathi1, Ravi Upadhyay2

1Sri Satya Sai University of Technology and Medical Sciences, Sehore-466001 (M.P.), India

2Government PG College, Pipariya-461775 (M.P.), India

Abstract

Large quantities of kitchen waste are produced in modern society and its disposal poses serious environmental and social problems. The production of organic compound from the kitchen waste by microflora can minimize the kitchen waste toxin and environmental pollution. The organic compound are used in the food and beverages industries, pharmaceutical industries, cosmetics and biopolymer production. It was intended to isolate and partial identification of bacteria and fungi from fermentation of vegetable garbage of kitchen responsible for the biosynthesis of organic acid. The collected garbage was allowed to ferment for 2 months without any addition of nutritional supplements for the growth of natural microflora into the fermentating garbage. The liquid extract released during the process of fermentation was used to isolate various types of bacteria and fungi. The isolated bacteria and fungi were subjected to different test for partial identification of the microorganism. The outcomes of study indicates the presence Staphylococcus aureus, Staphylococcus epidermidis, Lactobacillus spp., Bacillus subtilis, Pseudomonas spp. and Escherichia coli, Aspergillus niger, Aspergillus flavus, Rhizopus oryza, Alternaria species and Saccharomyces cereviceae in the kitchen waste. The results of this study suggest that the microorganism assist the production of organic compound in the kitchen waste.   

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Cost Effective Analysis Between Glimipiride and Gliptin Used in Diabetic Patients in Tertiary Care Hospital

 

Bharathi DR1 , Abubaker Siddiq1*, Ancy Jose George2

1Department of Pharmacology, SJM College of Pharmacy, Chitradurga - 577502, Karnataka, India

2Department of Pharmacy Practice, SJM College of Pharmacy, Chitradurga - 577502, Karnataka, India

Abstract

Diabetes mellitus is a spectrum of common metabolic disorders arising from various mechanisms all resulting in hyperglycemia. It is a disorder which can lead to coronary artery disease, cerebro- vascular disease, Nephropathy, Diabetic retinopathy, Neuropathy. So, early treatment is necessary to prevent these complications. In this regard, Oral Hypoglycemic drugs are most common type of treatment given for patient. A better cost effective drug can minimize the economic burden on the patients as it is a chronic diseases. The present study was aimed to asses cost effectiveness between glimepride and gliptin that may improve the clinical and economical aspect of patient and increase quality of health in patient. The present study was made to conduct cost effective analysis between glimepride and gliptin. It is a prospective observational study conducted in Basaveshwara Medical College, Hospital and Research Centre and Chitradurga Diabetic Centre for a period of six months. Patients admitted in General medicine above the age of 30 yrs are included. The data was collected from medical records of the patients and documented in suitable designed form.A total of 104 patients were enrolled during the study period, 70 are inpatients and 34 are outpatients. 5 were dropped out from the study.  The results reveal that Glimepride has got more efficacy than gliptin. It provided long term Glycemic control than gliptin and also remain less costly compare to gliptin. The one sample student T-test results showed the P value was 0.000*. After follow up glimepride proved to be cost effective drug. Increased health expenditures have led to the need to find out the optimal therapy at the lowest price. This study concluded that Glimepride is more efficacious and cost effective drug than Gliptin. Since diabetes is a chronic disease with long duration, Glimepride might be preferred to Gliptin. It also reveal that glimepride leads to improvements in quality adjusted-life expectancy and is a cost-effective option for the treatment of Type 2 diabetes.

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