Frequency and Antifungal Susceptibility Pattern of Candida Species Causing Candidemia in Bone Marrow Transplant Unit

Dr. Wajid Hussain1, Saira Bashir2, Prof. Irfan Ali Mirza1, Dr. Anam Imtiaz1, Dr. Umar Khurshid1, Prof Humaira Zafar3*, Dr Mariam Sarwar1

1Department of Microbiology, Armed Forces Institute of Pathology (AFIP), Rawalpindi (46000), Pakistan
2National University of Medical Sciences (NUMS), Rawalpindi (46000), Pakistan
3Al Nafees Medical College & Hospital (44000), Islamabad

Received: 22-Nov-2020 , Accepted: 18-Jan-2021

Keywords: Anti-fungal susceptibility, Blood stream infections. Candidemia, Immunocompromised patients, Candida albicans

DOI: http://dx.doi.org/10.20510/ukjpb/9/i1/1611401197

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How to Cite this Article

Hussain W, Bashir S, Mirza IA, Imtiaz A, Khurshid U, Zafar H, Sarwar M. Frequency and Antifungal Susceptibility Pattern of Candida Species Causing Candidemia in Bone Marrow Transplant Unit.  Pharmaceutical and Biosciences Journal. 2021; 9(1): 45-49.

Abstract

To determine the frequency and antifungal susceptibility pattern of Candida species causing candidemia or blood stream infections (BSI) in immunocompromised patientsof a bone marrow transplant unit. The study was conducted at the Department of Microbiology, Armed Forces Institute of Pathology (AFIP), Rawalpindi in collaboration with Armed Forces Bone Marrow Transplant Center (AFBMTC), Rawalpindi, from 1st June 2019 to 31st December 2019.Atotal 256 paired blood culture samples from patients of all ages, irrespective of gender were collected during a period of seven months. The samples were processed as recommended Clinical & Laboratory Standard Institute (CLSI) guidelines. Antifungal susceptibility by break point MICs was performed through VITEK® 2 system (version: 08.01) for Fluconazole, Itraconazole and Amphotericin B.  Of the total 256 blood cultures, 46 (17.97%) were positive for Candida species. The susceptibility of C. albicans was 100% to Amphotericin B, 90% to Fluconazole and 10% to Itraconazole, while C. tropicalis showed sensitivity of 92%, 88% and 0% against Amphotericin B, Fluconazole and Itraconazole, respectively. C. parapsilosis was found sensitive to Amphotericin B while resistance to Fluconazole and Itraconazole.Frequency of candidemia among immunocompromised patients is 17.9%. C. tropicalis and C. albicans are the two most common Candida species involved in blood stream infections in our setup. Fluconazole and Amphotericin B, both were found susceptible and can be used as empirical therapy.